蜡梅SAMT基因在烟草中的遗传转化及其功能分枏�/title>

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蜡梅SAMT基因在烟草中的遗传转化及其功能分枏�/p>

庞宏�? 向林, 赵凯�? 李响, 杨楠, 陈龙�? 蜡梅SAMT基因在烟草中的遗传转化及其功能分析[J]. 北京林业大学学报, 2014, 36(5): 117-122. doi: 10.13332/j.cnki.jbfu.2014.05.019

引用本文:

PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. doi: 10.13332/j.cnki.jbfu.2014.05.019

Citation:

PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J].Journal of Beijing Forestry University, 2014, 36(5): 117-122.doi:10.13332/j.cnki.jbfu.2014.05.019

引用本文:

Citation:

PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J].Journal of Beijing Forestry University, 2014, 36(5): 117-122.doi:10.13332/j.cnki.jbfu.2014.05.019

doi:10.13332/j.cnki.jbfu.2014.05.019

1 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验� 2 湖北省林业科学研究院 3 浙江省农业科学院园艺研究所

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- 刊出日期:2014-09-30

Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco

1 Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070,P. R. China;

摘要

摘要:为研究蜡梅SAMT 基因的调控功�?利用农杆菌介导法将其转入烟草中。经PCR 方法对转基因烟草进行检 �?结果显示:16 株转化植株中�?5 株扩增出了目的条�?进一步的RT-PCR 检测结果表�?阳性植株均发生了正确转录。对转基因烟草植株和未转基因对照植株进行植株大小、叶片形态、花色、花瓣大小以及花期进行观�?� 未发现有明显差异。采用顶空固相微萃取以及气相色谱-质谱技�?HS-SPME-GC-MS)对转基因烟草鲜花进行� 香成分分�?结果表明,转CpSAMT 基因烟草中有较高含量的苯甲醇、己烯醇、芳樟醇、石竹烯和苯甲醛等成�?但未能检测到水杨酸甲酯和苯甲酸甲酯成分、�p style="line-height: 12px;">

关键诌�

Abstract:In order to investigate the biological function of Chimonanthus praecox SAMT gene, CpSAMT was introduced into tobacco through Agrobacterium tumefaciens mediated transformation. Among 16 independent transgenic lines obtained, 15 lines were positive, which was revealed by PCR and further confirmed by RT-PCR. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. In addition, HS-SPME-GC-MS was employed to analyse the components of flower flavor. We found that the content of benzyl alcohol, hexenol, linalool, caryophyllene and benzaldehyde in transgenic CpSAMT plants increased, while that of methyl salicylate and methyl benzoate was undetectable compared with non-transgenic controls.

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