基金项目:国家自然科学基金青年科学基金项目?2201594),河北省教育厅科学技术研究项目(QN2022017),河北省省属高校基本科研业务费研究项目(KY2021059),河北省引进留学人员资助项目(C20210114),国家重点研发计划课题?019YFD1001605)、/div>
详细信息
高梦娇。主要研究方向:枣树逆境生理与分子生物技术。Email9a href="//www.inggristalk.com/j/article/doi/10.12171/mailto:mjgaomengjiao@163.com">mjgaomengjiao@163.com 地址?71000 河北省保定市莲池区乐凯南大街2596号河北农业大学西校区
王立新,博士,讲师。主要研究方向:枣树逆境生理与分子生物技术。Email9a href="//www.inggristalk.com/j/article/doi/10.12171/mailto:wanglxht@163.com">wanglxht@163.com 地址:同三/span>
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出版历程
- 收稿日期:2021-08-28
- 录用日期:2023-01-13
- 修回日期:2021-10-22
- 网络出版日期:2023-01-14
- 刊出日期:2023-03-25
Molecular characteristics ofCMLgenes in Chinese jujube and their expression patterns in resistance to cold stress
- Gao Mengjiao1,,
- Wang Linxia1,
- Luo Zhi1,
- Zhao Ruoyu1,
- Liu Zhiguo1, 2,
- Liu Ping1, 2,
- Liu Mengjun1, 2,
- Wang Lixin1, 2,,
- 1.
College of Horticulture, Hebei Agricultural University, Baoding 071001, Hebei, China
- 2.
Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, Hebei, China
摘要:
目的探究枣类钙调蛋白(ZjCML)在抗寒性中的重要作用,旨在挖掘关键抗寒
ZjCML基因,为进一步利用其进行枣抗寒育种提供理论参考、/sec>
方法本研究利用生物信息学技术,全面分析了枣
CML基因家族成员的数目及相关结构特征,并利用RNA-seq和实时荧光定量PCR技术分析其响应低温胁迫时的表达模式,为筛选关键抗寒基因奠定基础、/sec>
结果在枣基因组数据中共鉴定到23?i>ZjCML基因,不均匀地分布在12条染色体上。与拟南芥CMLs蛋白构建进化树分析发现ZjCMLs可被分为12个类群。蛋白网络互作预测发?6个ZjCMLs存在于该网络中,且具有一定的互作关系。RNA-seq结果表明
ZjCML13咋i>ZjCML6基因表达模式在‘冬枣’及其同源四倍体响应低温胁迫中具有显著差异,其中
ZjCML13在‘冬枣’响应低温胁??4 h时极显著上调表达,而在‘冬枣’同源四倍体中表达差异不显著。外源CaCl
2和褪黑素处理后,
ZjCML13在‘冬枣’同源四倍体响应低温胁迫6?4 h时极显著上调表达,说明其可能通过诱导
ZjCML13表达调控‘冬枣’同源四倍体抗寒性、/sec>
结论枢i>CML基因家族具有特定的结构特征及响应低温胁迫+i>ZjCML13可能在调控‘冬枣’及其同源四倍体抗寒性差异中发挥重要作用、/sec>
Abstract:
ObjectiveThis paper aims to investigate the important founction of CaM-like proteins in Chinese jujube in resistance to cold stress and identify the key
ZjCML, which could provide theoretical basis for cold breeding in Chinese jujube by using them.
MethodThrough using bioinformatics technology, the number and related structural information of
CMLin Chinese jujube were analyzed comprehensively in the current study. And then, we used RNA-seq and real-time fluorescence quantitative PCR technology to analyze its expression pattern in response to low temperature stress, laying a foundation for screening key cold resistance genes.
ResultA total of 23
ZjCMLgenes were identified in Chinese jujube genome. All of them distributed unevenly on 12 chromosomes. In addition,
ZjCMLcan be divided into 12 subgroups, and protein interaction network prediction showed that 16 ZjCML existed in the network and they had mutual interaction relationships. Moreover, RNA-seq analysis showed that the expression patterns of
ZjCML13 and
ZjCML6 in response to cold stress between ‘Dongzao and its autotetraploids were significantly different. The expression level of
ZjCML13 in response to cold stress in ‘Dongzao was significantly higher than that in its autotetraploid at 6 and 24 h. External CaCl
2and melatonin treatments could significantly induce the expression of
ZjCML13 in autotetraploids ‘Dongzao under cold stress at 6 and 24 h, indicating that it may regulate cold resistance of autotetraploid ‘Dongzao by inducing the expression of
ZjCML13.
ConclusionThe
ZjCMLgene family have specific characteristics and expression patterns,
ZjCML13 might play important role in regulation of cold difference between ‘Dongzao and its autotetraploids.
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