摘要: 目的为筛选出抗灰霉病能力强的亚洲百合品种，对部分Tiger、Pearl系列亚洲百合品种及兰州百合进行灰霉病抗性评价并初步探究其抗病的生理机制、�/sec> 方法对不同品种亚洲百合进行离体叶片接菌，评价其相对抗病能力，并测定筛选出的抗病和感病品种接菌后的生理变化、�/sec> 结果�?）Tiger、Pearl系列百合品种及兰州百合对灰霉病的抗性存在差异。其中抗灰霉病能力相对较强的品种是‘Pearl Melanie’，属于中抗水平；抗性最弱的是‘Pearl Justin’、兰州百合、‘Pearl White’和‘White Twinkle’。（2）供试百合的抗性水平与其气孔密度、气孔大小无显著相关性。（3）相对抗病的品种‘Pearl Melanie’和感病品种‘Pearl Justin’在接菌后的生理响应显著不同。随着接菌时间的延长，丙二醛的含量总体呈增加趋势，草酸氧化酶和几丁质酶的活性逐渐增强，�?1,3-葡聚糖酶的活性随时间的延长呈下降趋势。其中抗病品种的丙二醛含量相对低于感病品种，草酸氧化酶和几丁质酶的活性高于感病品种、�/sec> 结论叶片气孔的性状与抗病能力无显著相关性，抗病能力不同的百合在生理机制上有一定差异。在生理响应过程中，丙二醛含量、草酸氧化酶活性和几丁质酶活性可作为筛选亚洲百合对灰霉病抗性的评价指标、�/sec>

关键诌�
• 亚洲百合/
• 灰霉菋�/a> /
• 抗性评件�/a> /
• 生理响应

Abstract: ObjectiveThis paper aims to select Asiatic lilies with strong resistance to Botrytis elliptica, the resistance of some Tiger and Pearl series Asian lilies and Lilium davidiivar. unicolorto B. ellipticawas evaluated, and the physiological mechanism of resistance was preliminarily explored. MethodThe disease resistance of different cultivars or species was evaluated by ex vivo leaf inoculation. On this basis, the physiological changes of selected disease-resistant and susceptible cultivars after inoculation were determined. ResultThe comprehensive evaluation results showed that: (1) the resistance of Tiger, Pearl lilies and L. davidiivar. unicolorto B. ellipticawas different. Relatively strong resistance to B. ellipticawas ‘Pearl Melanie�? which belonged to the medium resistance level. The weakest resistance materials were ‘Pearl Justin�? L. davidiivar. unicolor, ‘Pearl White� and ‘White Twinkle�? (2) There was no significant correlation between resistance level and stomatal density, stomatal size. (3) The physiological responses of the resistant cultivar ‘Pearl Melanie� and susceptible cultivar ‘Pearl Justin� were obviously different after inoculation. The content of MDA showed an increasing trend with the extension of inoculation time, while the activities of oxalate oxidase and chitinase gradually increased, and the activities of β-1,3-glucanase decreased with time. The MDA content of the resistant cultivars was relatively lower than susceptible cultivar, and the activities of oxalate oxidase and chitinase were higher than susceptible cultivar. ConclusionThere is no significant correlation between stomatal characteristics and disease resistance, the physiological mechanism of lillies with distinct disease resistance is different. During the physiological response, MDA content, oxalate oxidase activity and chitinase activity can be used as evaluation indexes for evaluating Asiatic lilies for resistance to B. elliptica.

Key words:
• Asiatic lily/
• Botrytis elliptica/
• resistance evaluation/
• physiological response

�?nbsp; 1椭圆葡萄孢菌的培养（A）及菌丝体观察（B（�/p>

Figure 1.Culture ofB. elliptica(A) and observation of mycelium (B)

下载: 全尺寸图牆�/a> 幻灯牆�/a>

�?nbsp; 2不同品种/种百合接菌后5 d内的发病率变匕�/p>

Figure 2.Changes of incidence rate of different varieties/species of lily within 5 d after inoculation

下载: 全尺寸图牆�/a> 幻灯牆�/a>

�?nbsp; 3不同抗性百合的叶片气孔观察

Figure 3.Observation on leaf stomata ofLiliumwith different resistance

下载: 全尺寸图牆�/a> 幻灯牆�/a>

�?nbsp; 4‘马尼拉/光芒’和‘贾斯汀’叶片接菌后的病斑变匕�/p>

Figure 4.Lesion changes on leaves of ‘Pearl Melanie� and ‘Pearl Justin� after inoculation

下载: 全尺寸图牆�/a> 幻灯牆�/a>

�?nbsp; 5‘马尼拉/光芒’和‘贾斯汀’叶片接菌后的生理响库�/p>

Figure 5.Physiological response of ‘Pearl Melanie� and ‘Pearl Justin� leaves toB. ellipticaafter inoculation

下载: 全尺寸图牆�/a> 幻灯牆�/a>

�?nbsp; 1供试材料

Table 1.Test materials

品种/种名 Cultivar/species name	花色 Flower color
‘美人� ‘Pearl Carolina‘�/td>	橙红 Red orange
‘马尼拉/光芒� ‘Pearl Melanie‘�/td>	黄白 Light white
‘雷恩� ‘Pearl Loraine‘�/td>	� Red
‘红旗� ‘Red Life‘�/td>	深红 Crimson
‘幼虎� ‘Tiger Babies‘�/td>	黄赭 Yellow ochre
‘璀璨� ‘Pearl Stacey‘�/td>	铜黄 Bronze yellow
‘贾斯汀� ‘Pearl Justin‘�/td>	� Yellow
‘红色的天鹅绒� ‘Red Velvet‘�/td>	赭红 Ochre red
‘粉色飞行� ‘Pink Flight‘�/td>	亮粉 Bright pink
‘白色闪耀� ‘White Twinkle‘�/td>	� White
‘白珍珠� ‘Pearl White‘�/td>	米白 Off-white
兰州百合Lilium davidiivar.unicolor	� Orange

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�?nbsp; 2叶部病斑分级标准

Table 2.Grading standard of leaf lesions

病级 Disease grade	分级标准 Grading standard
0	无病� No lesions
1	叶片病斑较少，病斑面积占整个叶面积的5%以下 Fewer leaf lesions, accounting for less than 5% of the entire leaf area
3	病斑较多，病斑面积占整个叶面积的5% ~ 30% More lesions, accounting for 5% to 30% of the entire leaf area
5	病斑较多，病斑面积占整个叶面积的31% ~ 50% More lesions, accounting for 31% to 50% of the entire leaf area
7	病斑很多或者融合成大斑，病斑面积占整个面积�?0%以上 Many lesions or fused into large lesions, accounting for more than 50% of the entire leaf area

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�?nbsp; 3不同百合离体接种条件下的灰霉菌抗性鉴宙�/p>

Table 3.Identification of resistance toB. ellipticaunder ex vivo inoculation conditions of differentLiliumcultivars

�?品种 Species/cultivar	发病� Incidence rate	相对病情指数 Relative disease index	相对抗病指数 Relative resistance index	抗性水� Resistance level
兰州百合L. davidiivar.unicolor	0.54	0.89	0.11	HS
‘美人� ‘Pearl Carolina‘�/td>	0.49	0.91	0.09	HS
‘马尼拉/光芒� ‘Pearl Melanie‘�/td>	0.17	0.39	0.61	R
‘雷恩� ‘Pearl Loraine‘�/td>	0.31	0.71	0.29	HS
‘红旗� ‘Red Life‘�/td>	0.39	0.59	0.41	S
‘幼虎� ‘Tiger Babies‘�/td>	0.33	0.48	0.52	S
‘璀璨� ‘Pearl Stacey‘�/td>	0.34	0.79	0.21	HS
‘贾斯汀� ‘Pearl Justin‘�/td>	0.56	0.99	0.01	HS
‘红色的天鹅绒� ‘Red Velvet‘�/td>	0.39	0.85	0.15	HS
‘粉色飞行� ‘Pink Flight‘�/td>	0.39	0.63	0.37	S
‘白色闪耀� ‘White Twinkle‘�/td>	0.52	0.92	0.08	HS
‘白珍珠� ‘Pearl White‘�/td>	0.53	0.97	0.03	HS
注：R. 中抗；S. 中感� HS. 高感。下同。Notes: R, moderately resistant; S, moderately susceptible; HS, highly susceptible. The same below.

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�?nbsp; 4不同抗性百合气孔特�?/p>

Table 4.Stomatal traits ofLiliumwith different resistance

�?品种 Species/cultivar	气孔长度 Stoma length/μm	气孔宽度 Stoma width/μm	气孔长宽毓�br/>Stoma length-width ratio	气孔密度/（个·mm�?（�br/>Stoma density/(number·mm�?)	抗性水干�br/>Resistance level
兰州百合L. davidiivar.unicolor	62.95 ± 2.01	32.04 ± 1.31	1.96	87.46 ± 4.93	HS
‘美人� ‘Pearl Carolina‘�/td>	84.07 ± 2.57	48.99 ± 1.66	1.72	35.55 ± 2.14	HS
‘马尼拉/光芒� ‘Pearl Melanie‘�/td>	84.95 ± 5.08	41.94 ± 2.19	2.03	30.87 ± 1.40	R
‘雷恩� ‘Pearl Loraine‘�/td>	74.12 ± 3.50	36.83 ± 1.95	2.01	48.17 ± 7.98	HS
‘红旗� ‘Red Life‘�/td>	100.67 ± 2.49	46.53 ± 2.87	2.16	36.01 ± 2.92	S
‘幼虎� ‘Tiger Babies‘�/td>	78.16 ± 5.55	38.31 ± 1.37	2.04	69.69 ± 2.14	S
‘璀璨� ‘Pearl Stacey‘�/td>	69.85 ± 4.14	41.93 ± 1.81	1.67	59.40 ± 2.14	HS
‘贾斯汀� ‘Pearl Justin‘�/td>	91.85 ± 5.22	46.95 ± 2.18	1.96	26.66 ± 2.43	HS
‘红色的天鹅绒� ‘Red Velvet‘�/td>	87.71 ± 3.73	41.57 ± 2.80	2.11	43.96 ± 0.81	HS
‘粉色飞行� ‘Pink Flight‘�/td>	102.28 ± 5.43	48.59 ± 1.95	2.10	42.56 ± 2.14	S
‘白色闪耀� ‘White Twinkle‘�/td>	62.32 ± 2.82	37.74 ± 2.58	1.65	58.46 ± 3.53	HS
‘白珍珠� ‘Pearl White‘�/td>	86.89 ± 1.95	38.90 ± 3.98	2.23	43.50 ± 5.06	HS

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�?nbsp; 5不同材料的气孔性状与抗性之间的相关�?/p>

Table 5.Correlation between stomatal traits and resistance of different materials

气孔性状 Stomatal trait	指标 Index	气孔长度 Stoma length	气孔宽度 Stoma width	气孔密度 Stoma density	抗性水干�br/>Resistance level
气孔长度 Stoma length	r	1	0.781**	�?.729**	�?.416
	P		0.003	0.007	0.178
气孔宽度 Stoma width	r	0.781**	1	�?.774**	�?.229
	P	0.003		0.003	0.474
气孔密度 Stoma density	r	�?.729**	�?.774**	1	0.253
	P	0.007	0.003		0.427
抗性水干�br/>Resistance level	r	�?.416	�?.229	0.253	1
	P	0.178	0.474	0.427
注：**表示�?.01水平上极显著相关。Note: ** indicates a very significant correlation at 0.01 level.

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