doi:10.12171/j.1000-1522.20210150
Study on construction method of microcore germplasm ofPopulus tomentosa
?nbsp; 1产地样本数及无性系编号
Table 1.Sample number from provenances and clone No.
产地 Provenance |
样本?br/> Sample number | 无性系编号 Clone No. |
北京 Beijing | 25 | 1 ~ 25 |
河北 Hebei | 56 | 26 ~ 81 |
山东 Shandong | 19 | 82?3?21?22?32?34?35?37?42 ~ 249?51?54?55 |
河南 Henan | 41 | 84 ~ 114?23?25 ~ 230?57 ~ 259 |
山西 Shanxi | 44 | 115 ~ 157?60 |
陕西 Shaanxi | 49 | 158 ~ 203?38?39?61 |
甘肃 Gansu | 5 | 204 ~ 208 |
安徽 Anhui | 9 | 209 ~ 217 |
江苏 Jiangsu | 4 | 218 ~ 220?63 |
?nbsp; 2毛白杨核心种质排序表
Table 2.Rank of core germplasm ofP. tomentosa
顺序 Order |
无性系编号 Clone No. |
顺序 Order |
无性系编号 Clone No. |
顺序 Order |
无性系编号 Clone No. |
顺序 Order |
无性系编号 Clone No. |
|||
1 | 156 | 18 | 265 | 35 | 227 | 52 | 161 | |||
2 | 258 | 19 | 263 | 36 | 264 | 53 | 113 | |||
3 | 236 | 20 | 233 | 37 | 211 | 54 | 160 | |||
4 | 253 | 21 | 225 | 38 | 196 | 55 | 259 | |||
5 | 266 | 22 | 267 | 39 | 208 | 56 | 203 | |||
6 | 262 | 23 | 218 | 40 | 176 | 57 | 158 | |||
7 | 261 | 24 | 222 | 41 | 195 | 58 | 110 | |||
8 | 231 | 25 | 270 | 42 | 138 | 59 | 155 | |||
9 | 34 | 26 | 197 | 43 | 193 | 60 | 272 | |||
10 | 241 | 27 | 256 | 44 | 152 | 61 | 202 | |||
11 | 271 | 28 | 269 | 45 | 184 | 62 | 154 | |||
12 | 242 | 29 | 220 | 46 | 180 | 63 | 255 | |||
13 | 252 | 30 | 250 | 47 | 181 | 64 | 151 | |||
14 | 186 | 31 | 191 | 48 | 89 | 65 | 201 | |||
15 | 240 | 32 | 219 | 49 | 26 | 66 | 47 | |||
16 | 237 | 33 | 72 | 50 | 178 | 67 | 147 | |||
17 | 268 | 34 | 215 | 51 | 126 | 68 | 109 |
?nbsp; 3不同取样比例种质总数及不同来源份?/p>
Table 3.Total number of germplasms with different sampling ratios and the number of samples from different sources
比例 Ratio/% |
北京 Beijing |
河北 Hebei |
山东 Shandong |
河南 Henan |
山西 Shanxi |
陕西 Shaanxi |
甘肃 Gansu |
安徽 Anhui |
江苏 Jiangsu |
新疆 Xinjiang |
杂种 Hybrid |
总计 Total |
100.0 | 25 | 56 | 19 | 41 | 44 | 49 | 5 | 9 | 4 | 1 | 19 | 272 |
25.0 | 0 | 4 | 4 | 8 | 8 | 18 | 1 | 2 | 4 | 1 | 18 | 68 |
20.0 | 0 | 3 | 3 | 6 | 4 | 14 | 1 | 2 | 4 | 1 | 17 | 55 |
15.0 | 0 | 2 | 3 | 3 | 1 | 7 | 1 | 2 | 4 | 1 | 17 | 41 |
10.0 | 0 | 1 | 3 | 2 | 1 | 3 | 0 | 0 | 2 | 1 | 14 | 27 |
5.0 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 8 | 14 |
2.5 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 7 |
2.0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
1.5 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
?nbsp; 4不同取样比例微核心种质遗传多样性分枏/p>
Table 4.Genetic diversity analysis of microcore germplasm with different sampling ratios
样本野br/> Sample number (N) |
取样比例 Sampling ratio/% |
等位基因?br/> Allele number (Na) |
有效等位基因?br/> Effective number of allele (Ne) |
Shannon信息指数 Shannon information index (I) |
观测杂合?br/> Observed heterozygosity (Ho) |
期望杂合?br/> Expected heterozygosity (He) |
平均多态信息指?br/>Average polymorphic information index (PIC) |
272 | 100.0 | 6.625 | 2.075 | 0.825 | 0.561 | 0.432 | 0.364 |
68 | 25.0 | 6.063 | 2.761 | 1.094 | 0.605 | 0.539 | 0.499 |
55 | 20.0 | 6.063 | 2.916 | 1.141 | 0.605 | 0.557 | 0.519 |
41 | 15.0 | 5.875 | 3.145 | 1.205 | 0.601 | 0.584 | 0.547 |
27 | 10.0 | 5.813 | 3.317 | 1.271 | 0.581 | 0.614 | 0.578 |
14 | 5.0 | 4.938 | 3.385 | 1.234 | 0.589 | 0.616 | 0.576 |
7 | 2.5 | 4.625 | 3.404 | 1.254 | 0.554 | 0.638 | 0.594 |
5 | 2.0 | 4.438 | 3.513 | 1.254 | 0.550 | 0.643 | 0.597 |
4 | 1.5 | 3.625 | 3.003 | 1.048 | 0.625 | 0.563 | 0.512 |
?nbsp; 5微核心种质(10%)与初始种质的遗传多样性比辂/p>
Table 5.Comparison of genetic diversity between microcore germplasm (10%) and primary germplasm
群体 Population | N | Na | Ne | I | Ho | He | PIC |
初始种质 Primary germplasm | 272 | 6.625 | 2.075 | 0.825 | 0.561 | 0.432 | 0.364 |
微核心种 Microcore germplasm | 27 | 5.813 | 3.317 | 1.271 | 0.581 | 0.614 | 0.578 |
保留 Retention rate/% | 10 | 87.743 | 159.855 | 154.061 | 103.565 | 142.130 | 158.791 |
t | 0.993 8 | ||||||
P | 0.358 7 > 0.05 |
?nbsp; 6微核心种质(2%)与初始种质的遗传多样性比辂/p>
Table 6.Comparison of genetic diversity between the microcore germplasm (2%) and primary germplasm
群体 Population | N | Na | Ne | I | Ho | He | PIC |
初始种质 Primary germplasm | 272 | 6.625 | 2.075 | 0.825 | 0.561 | 0.432 | 0.364 |
微核心种 Microcore germplasm | 5 | 4.438 | 3.513 | 1.254 | 0.550 | 0.643 | 0.597 |
保留 Retention rate/% | 2 | 66.989 | 169.301 | 152.000 | 98.039 | 148.843 | 164.011 |
t | 0.999 4 | ||||||
P | 0.356 2 > 0.05 |
?nbsp; 7微核心种质(15%)与初始种质的遗传多样性比辂/p>
Table 7.Comparison of genetic diversity between the microcore germplasm (15%) and primary germplasm
群体 Population | N | Na | Ne | I | Ho | He | PIC |
初始种质 Primary germplasm | 272 | 6.625 | 2.075 | 0.825 | 0.561 | 0.432 | 0.364 |
微核心种 Microcore germplasm | 41 | 5.875 | 3.145 | 1.205 | 0.601 | 0.584 | 0.547 |
保留 Retention rate/% | 15 | 88.679 | 151.566 | 146.061 | 107.130 | 135.185 | 150.275 |
t | 0.994 6 | ||||||
P | 0.358 4 > 0.05 |
?nbsp; 8微核心种质(1.5%)与初始种质遗传多样性比辂/p>
Table 8.Comparison of genetic diversity between the microcore germplasm (1.5%) and primary germplasm
群体 Population | N | Na | Ne | I | Ho | He | PIC |
初始种质 Primary germplasm | 272 | 6.625 | 2.075 | 0.825 | 0.561 | 0.432 | 0.364 |
微核心种 Microcore germplasm | 4 | 3.625 | 3.003 | 1.048 | 0.625 | 0.563 | 0.512 |
保留 Retention rate/% | 1.5 | 54.717 | 144.723 | 127.030 | 111.408 | 130.324 | 140.659 |
t | 1.006 5 | ||||||
P | 0.353 0 > 0.05 |
?nbsp; 916个SSR位点27份微核心种质的多态性分枏/p>
Table 9.Polymorphism analysis of 27 accessions of microcore germplasm at 16 SSR locus
引物 Primer |
位点 Locus |
N | Na | Ne | I | Ho | He | PIC |
1 | Ptr_1_SSR2 | 27 | 5.000 | 3.291 | 1.300 | 0.593 | 0.696 | 0.640 |
2 | Ptr_7_SSR14 | 27 | 7.000 | 4.500 | 1.649 | 0.815 | 0.778 | 0.744 |
3 | Ptr_1_SSR1 | 27 | 3.000 | 1.573 | 0.655 | 0.333 | 0.364 | 0.327 |
4 | Ptr_9_SSR3 | 27 | 4.000 | 2.282 | 1.040 | 0.222 | 0.562 | 0.512 |
5 | Ptr_11_SSR1 | 27 | 7.000 | 4.084 | 1.595 | 0.556 | 0.755 | 0.718 |
6 | Ptr_10_SSR1 | 27 | 4.000 | 2.809 | 1.136 | 0.852 | 0.644 | 0.574 |
7 | Ptr_11_SSR8 | 27 | 5.000 | 1.421 | 0.653 | 0.148 | 0.296 | 0.283 |
8 | Ptr_14_SSR12 | 27 | 7.000 | 4.166 | 1.636 | 0.852 | 0.760 | 0.726 |
9 | Ptr_14_SSR7 | 27 | 5.000 | 3.827 | 1.465 | 0.852 | 0.739 | 0.698 |
10 | Ptr_16_SSR3 | 27 | 6.000 | 1.369 | 0.651 | 0.111 | 0.270 | 0.263 |
11 | Ptr_3_SSR13 | 27 | 7.000 | 3.044 | 1.364 | 0.259 | 0.671 | 0.615 |
12 | Ptr_14_SSR11 | 27 | 6.000 | 2.553 | 1.239 | 0.778 | 0.608 | 0.566 |
13 | Ptr_18_SSR17 | 27 | 10.000 | 6.568 | 2.032 | 0.815 | 0.848 | 0.830 |
14 | Ptr_7_SSR15 | 27 | 9.000 | 6.688 | 2.015 | 0.926 | 0.850 | 0.833 |
15 | Ptr_13_SSR6 | 27 | 5.000 | 3.539 | 1.401 | 0.889 | 0.717 | 0.672 |
16 | Ptr_19_SSR1 | 27 | 3.000 | 1.353 | 0.503 | 0.296 | 0.261 | 0.241 |
平均 Mean | 27 | 5.813 | 3.317 | 1.271 | 0.581 | 0.614 | 0.578 |
?nbsp; 10?对引物构建的27份毛白杨微核心种质指纹图谰/p>
Table 10.Fingerprint map of 27 microcore germplasms ofP. tomentosaconstructed using the top 8 pairs of primers
顺序 Order |
无性系编号 Clone No. |
引物 Primer | |||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
1 | 156 | 378/378 | 393/396 | 185/185 | 369/372 | 271/281 | 241/244 | 332/332 | 333/345 |
2 | 258 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
3 | 236 | 374/382/390 | 360/363/399 | 185/185 | 369/369 | 269/283 | 238/241 | 332/332 | 335/337 |
4 | 253 | 378/378 | 360/396 | 185/185 | 372/372 | 271/287 | 238/241 | 294/294 | 333/337 |
5 | 266 | 390/390 | 357/363 | 183/183 | 269/269 | 275/275 | 238/238 | 330/330 | 333/333 |
6 | 262 | 374/378 | 360/396 | 185/185 | 372/372 | 271/283 | 241/244 | 332/332 | 333/333 |
7 | 261 | 382/390 | 363/396 | 185/187 | 269/269 | 269/269 | 238/241/250 | 332/332 | 337/337 |
8 | 231 | 376/378 | 393/396 | 185/185 | 369/372 | 271/271 | 241/244 | 332/332 | 333/345 |
9 | 34 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
10 | 241 | 378/378 | 393/396 | 185/185 | 372/372 | 271/271 | 238/244 | 332/332 | 333/353 |
11 | 271 | 374/382/390 | 360/363/399 | 185/185 | 369/369 | 269/269 | 238/241 | 332/332 | 333/337 |
12 | 242 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
13 | 252 | 378/378 | 396/396 | 185/185 | 369/372 | 271/281 | 241/244 | 332/332 | 333/345 |
14 | 186 | 382/390 | 363/396 | 185/187 | 369/369 | 269/269 | 238/241/250 | 332/332 | 335/337 |
15 | 240 | 378/378 | 393/396 | 185/185 | 372/372 | 271/271 | 238/244 | 332/332 | 333/353 |
16 | 237 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
17 | 268 | 378/378 | 360/396 | 185/185 | 369/372 | 271/271 | 241/244 | 332/332 | 333/345 |
18 | 265 | 378/382 | 360/399 | 185/185 | 372/372 | 283/287 | 238/241 | 294/332 | 333/337 |
19 | 263 | 382/390 | 360/363 | 185/185 | 372/372 | 269/281 | 238/250 | 332/338 | 333/337 |
20 | 233 | 378/378 | 396/396 | 185/185 | 366/369 | 271/271 | 241/241 | 332/332 | 331/333 |
21 | 225 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
22 | 267 | 390/390 | 363/363 | 185/187 | 269/269 | 285/285 | 238/238 | 330/334 | 333/333 |
23 | 218 | 378/378 | 360/360 | 185/185 | 366/366 | 271/271 | 241/241 | 332/332 | 331/353 |
24 | 222 | 382/390 | 363/366/399 | 185/187 | 372/372 | 269/283 | 238/241/244 | 332/332 | 337/355 |
25 | 270 | 378/378 | 396/396 | 185/185 | 369/372 | 271/271 | 241/244 | 332/332 | 333/345 |
26 | 197 | 382/390 | 360/363 | 185/185 | 372/372 | 269/281 | 238/250 | 332/332 | 333/337 |
27 | 256 | 378/382/390 | 360/363/399 | 185/185 | 372/372 | 271/283 | 238/241 | 294/332 | 335/337 |
?nbsp; 11?对引物构建的27份毛白杨微核心种质指纹图谰/p>
Table 11.Fingerprint map of 27 microcore germplasms ofP.tomentosa constructed using the last 8 pairs of primers
顺序 Order |
无性系编号 Clone No. |
引物 Primer | |||||||
9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | ||
1 | 156 | 253/262 | 356/356 | 373/373 | 327/348 | 177/179 | 347/355 | 339/345 | 289/292 |
2 | 258 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/342/348 | 289/289 |
3 | 236 | 259/259 | 356/356 | 353/353 | 327/327 | 171/181 | 355/361/363 | 342/345 | 280/289 |
4 | 253 | 253/259 | 356/356 | 373/373 | 327/336 | 173/177 | 339/365 | 339/339 | 289/289 |
5 | 266 | 259/259 | 340/340 | 371/371 | 321/330 | 193/203 | 363/363 | 336/342 | 289/289 |
6 | 262 | 262/262 | 356/356 | 353/353 | 327/327 | 179/181 | 339/365 | 336/345 | 280/289 |
7 | 261 | 256/259 | 356/356 | 353/353 | 327/336 | 171/173/175 | 359/363 | 339/342 | 280/289 |
8 | 231 | 253/262 | 356/356 | 373/373 | 327/348 | 177/181 | 347/355 | 339/345 | 289/292 |
9 | 34 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/348 | 289/289 |
10 | 241 | 253/259 | 356/356 | 375/375 | 306/327 | 173/173 | 347/373 | 336/342 | 289/289 |
11 | 271 | 256/262 | 356/356 | 353/353 | 327/327 | 171/171 | 355/361/363 | 342/345 | 280/289 |
12 | 242 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/342/348 | 289/289 |
13 | 252 | 253/262 | 356/356 | 373/373 | 327/348 | 177/179 | 347/355 | 339/345 | 289/289 |
14 | 186 | 256/259 | 356/356 | 353/353 | 327/336 | 171/173/175 | 359/363 | 339/342 | 280/289 |
15 | 240 | 253/259 | 356/356 | 375/375 | 306/327 | 173/173 | 347/373 | 336/342 | 289/289 |
16 | 237 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/342/348 | 289/289 |
17 | 268 | 253/262 | 356/356 | 373/373 | 327/348 | 177/177 | 347/355 | 342/345 | 280/289 |
18 | 265 | 259/262 | 356/356 | 387/387 | 327/327 | 173/179 | 361/365 | 336/339 | 289/289 |
19 | 263 | 256/262 | 356/356 | 353/353 | 327/336 | 173/191 | 361/363 | 339/342 | 289/289 |
20 | 233 | 259/262 | 326/334 | 373/373 | 327/327 | 179/181 | 347/355 | 339/345 | 289/289 |
21 | 225 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/342/348 | 289/289 |
22 | 267 | 259/262 | 316/328 | 369/369 | 321/330 | 201/201 | 363/365 | 342/348 | 289/289 |
23 | 218 | 259/259 | 326/334 | 353/377 | 327/327 | 177/179 | 355/355 | 339/339 | 289/289 |
24 | 222 | 250/259 | 356/356 | 353/373 | 327/336 | 175/179 | 345/361/363 | 339/342/348 | 289/289 |
25 | 270 | 253/262 | 356/356 | 373/373 | 327/348 | 177/179 | 347/355 | 339/345 | 289/289 |
26 | 197 | 256/262 | 356/356 | 353/353 | 327/336 | 173/191 | 361/363 | 339/342 | 289/289 |
27 | 256 | 259/262 | 356/356 | 353/353 | 327/336 | 171/173 | 361/363/365 | 339/339 | 289/289 |