ࡱ> tvsg R}bjbj5{z\{z\[  66666JJJ84J=<<<<<<<$@9CJ=6=664=''' 66<'<''r799^L%8<=0=8C&C(99HC6J;'==*'j=C @ : Curriculum Vitae Personal Information Name: Inhwan Hwang; Position: Professor Affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, Korea, e-mail:ihhwng@postech.ac.kr Beijing Advanced Innovation Center, Beijing Forestry University, Beijing, China Interest of research areas 1. Intracellular trafficking in plant cells 2. Organelle biogenesis and communication between organelles 3. Cellular reprogramming 4. Development of plant cells as a bioreactor system 5. ABA metabolism and signaling International Academic Activity ; Editor and Editorial Board members `$ Plant and Cell Physiology Editorial Boarder member (2013.01.01-Current) a$ Frontiers in Plant Cell Biology Editor (2011.01.01- 2015. 12) b$ Journal of Plant Biology, Associate Editor (2004.01.01-Current) c$ Plant Cell, Reviewing Editor (2015. 1. 1 - Current) d$ Plant Cell Reporters, Associate Editor (2015. 7. 24 - Current) Publication list (from 2005 to current) 62. Lee H-Y, Bahn SC, Shin JS, Hwang I, Back K, Doelling JH, and Ruy SB (2005). Multiple forms of secretory phospholipase A2 in Plants. Progress Lipid Res 44:52-67. 63. Lim YG, Sohn EJ, Seo J, Lee K-J, Lee H-S, Hwang I, Sacher WM,and Oh B-H (2005). The crystal structure of mammalian bet3 reveals a novel mechanism for Golgi localization of the tethering factor TRAPP. Nature Structural and Mol. Biol. 12:38-45. 64. Kim H, Park M, Kim SJ and Hwang I (2005). Actin filaments play a critical role in vacuolar trafficking at the Golgi complex in plant cells. Plant Cell 17:888-902. 65. Joo JH, Yoo HJ, Hwang I, Lee JS, Nam KH, and Bae YS (2005). Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase. FEBS Lett. 579:1243-1248. 66. Park M, Lee D, Lee G-J, and Hwang I (2005). The Ring-H2 finger domain-bearing RMR protein functions as a cargo receptor for a protein trafficked to the protein storage vacuole. J Cell Biol. 170:757-767. 67. Bae HJ, Lee DS, and Hwang I (2006). Simultaneous targeting of a protein to chloroplasts and peroxisomes as an approach to express heterologous proteins at the high level. J Exp Bot, 57:161-169. 68. Lee DW, Lee S, Lee G-J, Lee K-H, and Hwang I (2006) Functional characterization of sequence motifs in the transit peptide of RbcS. Plant Physiol. 140:466-483. 69. Teng Y-S, Su Y-s, Chen K-J, Lee YJ, Hwang I, and Li H-m (2006). Tic21 is an essential translocon component for protein translocation across the chloroplast inner envelope membrane. Plant Cell 18: 2247-2257. 70. Song J, Lee MH, Lee G-J, Yoo CM, and Hwang I (2006). An Arabidopsis epsin homolog AtEpsin1 plays a critical role in vacuolar trafficking of soluble proteins via interaction with clathrin, AtVTI11, AP1 and AtVSR1. Plant Cell 18: 2258-2274. 71. Miao Y, Yan PK, Kim H, Hwang I, and Jiang L (2006). Localization of GFP fusions with the seven Arabidopsis sorting receptors to the prevacuolar compartments in BY-2 cells. Plant Physiol. 142:945-962. 72. Lee MH, Lee SH, Kim H, Jin JB, Kim DH, and Hwang I. (2006). A WD40 repeat protein, Arabidopsis Sec13 homolog 1, may play a role in vacuolar trafficking by controlling the membrane association of AtDRP2A. Mol Cells. 22:210-219. 73. Lee KH, Piao HL, Kim H-Y, Choi SM, Jiang F, Hartung W, Hwang I, Kwak JM, Lee I-J, and Hwang I. (2006). Activation of glucosidase via dehydration-induced polymerization rapidly increases active pools of abscisic acid. Cell. 126, 1109-1120. 74. Jung KH, Han MJ, Lee DY, Lee YS, Schreiber L, Franke R, Faust A, Yephremov A, Saedler H, Kim YW, Hwang I, and An G (2006). Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development. Plant Cell 18:3015-3032. 75. Dhonukshe P, Aniento F, Hwang I, Robinson DG, Mravec J, Stierhof YD, and Friml J. (2007). Clathrin-Mediated Constitutive Endocytosis of PIN Auxin Efflux Carriers in Arabidopsis. Curr Biol. 17:520-527. 76. Min MK, Kim SJ, Miao Y, Shin J, Jiang L, and Hwang I. (2007). Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells. Plant Physiol. 143:1601-1614. 77. Lee GJ, Kim H, Kang H, Jang M, Lee DW, Lee S, Hwang I. (2007). EpsinR2 interacts with clathrin, adaptor protein-3, AtVTI12, and phosphatidylinositol -3-phosphate. Implications for EpsinR2 function in protein trafficking in plant cells. Plant Physiol. 143: 1561-1575. 78. Lee Y, Kim YW, Jeon BW, Park KY, Suh SJ, Seo J, Kwak JM, Martinoia E, Hwang I, and Lee Y. (2007) Phosphatidylinositol 4,5-bisphosphate is important for stomatal opening. Plant J. 52: 803-816. 79. Bae W, Lee YJ, Kim S, Kim DH, Sohn EJ, and Hwang I (2008) AKR2A-mediated biogenesis of chloroplast outer membrane proteins is essential for chloroplast development. Nature Cell Biology 10: 200-207. 80. Lee Y, Kim ES, Choi Y, Hwang I, Staiger CJ, Chung YY, and Lee Y (2008) The Arabidopsis phosphatidylinositol 3-kinase is important for pollen development. Plant Physiol. 147:1886-1897. 81. Lee DW, Kim JK, Lee S, Choi S, Kim S, and Hwang I (2008) Arabidopsis nuclear-encoded plastid transit peptides contain multiple sequence subgroups with distinctive chloroplast-targeting sequence motifs. Plant Cell 20:1603-1622. 82. Choi AM, Lee SB, Cho SH, Hwang I, Hur C-G, and Suh MC (2008) Isolation and characterization of multiple abundant lipid transfer protein isoforms in developing sesame (Sesamum indicum L.) seeds. Plant Physiol Biochem 46, 127-139. 83. Hwang I (2008) Sorting and anterograde trafficking at the Golgi apparatus. Plant Physiology 148, 673-683. 84. Lee HK, Cho SK, Son O, Xu Z, Hwang I, and Kim WT (2009) Rma1H1, a RING membrane-anchor E3 ubiquitin ligase homolog, regulates aquaporin levels via ubiquitination as an adaptive response to dehydration stress. Plant Cell 21:622-641. 85. Agne B, Infanger S, Wang F, Hofstetter V, Rahim G, Martin M, Lee DW, Hwang I, Schnell D, and Kessler F (2009) A toc159 import receptor mutant, defective in hydrolysis of GTP, supports preprotein import into chloroplasts. J Biol Chem. 284:8670-8679. 86. Lee SB, Go YS, Bae H-J, Park JH, Cho SH, Lee DS, Park OK, Hwang I, and Suh MC (2009) Disruption of glycosylphosphatidylinositol anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicolar. Plant Physiol, 150:42-54. 87. Kikuchi S, Oishi M, Hirabayashi Y, Lee DW, Hwang I, and Nakai M (2009) A 1-megadalton translocation complex containing Tic20 and Tic21 mediates chloroplast protein import at the inner envelope membrane. Plant Cell 21:1781-1797. 88. Lee DW, Lee S, Oh YJ, and Hwang I (2009) Multiple sequence motifs in the RbcS transit peptide independently contribute to Toc159-dependent import of proteins into chloroplastS. Plant Physiol 151: 129-141. 89. Park KS, Lee J, Ahn SS, Byun YH, Seong BL, Baek YH, Song MS, Choi YK, Na YJ, Hwang I, Sung YC, and Lee CG (2009) Mucosal immunity induced by adenovirus-based H5N1 HPAI vaccine confers protection against a lethal H5N2 avian influenza virus challenge. Virology. 395:182-189. 90. Jeon Y, Hwang AR, Hwang I, and Pai HS (2009) Silencing of NbCEP1 encoding a chloroplast envelope protein containing 15 leucine-rich-repeats disrupts chloroplast biogenesis in Nicotiana benthamiana. Mol Cells. 29:175-183. 91. Hwang I, and Robinson DG (2009) Transport vesicle formation in plant cells. Curr Opin Plant Biol. 12:660-669. 92. Lee S, Lee DW, Lee Y, Mayer U, Steirhof Y-D, Lee S, Jurgens G, and Hwang I (2009) Heat shock protein cognate 70-4 and an E3 Ubiquitin Ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis. Plant Cell 21:3984-4001. 93. Kim H, Kang H, Jang M, Chang JH, Miao Y, Jiang L, and Hwang I (2010) Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor. Plant Physiol. 154:134-48. 94. Jeon Y, Hwang AR, Hwang I, and Pai HS (2010) Silencing of NbCEP1 encoding a chloroplast envelope protein containing 15 leucine-rich-repeats disrupts chloroplast biogenesis in Nicotiana benthamiana. Mol Cells, 29, 175-183. 95. Kim MJ, Go YS, Lee SB, Kim YS, Shin JS, Min MK, Hwang I, and Suh MC (2010) Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor. Plant Mol Biol. 73:425-437. 96. Zhao Y, Yan A, Feijo JA, Furutani M, Takenawa T, Hwang I, Fu Y, and Yang Z (2010) Phosphoinositides Regulate Clathrin-dependent endocytosis at the Tip of Pollen Tubes in Arabidopsis Tobacco. Plant Cell 22:4031-4044. 97. Dong CH, Jang M, Scharein B, Malach A, Rivarola M, Liesch J, Groth G, Hwang I, and Chang C (2010) Molecular Association of the Arabidopsis ETR1 Ethylene Receptor and a Regulator of Ethylene Signaling, RTE1. J Biol Chem. 285: 40706-40713. 98. Jung CJ, Lee MH, Min MK, and Hwang I (2011) Localization and trafficking of an isoform of the AtPRA1 family to the Golgi apparatus depend on both N- and C-terminal sequence motifs. Traffic. 12:185-200. 99. Lee J, Lee H, Kim J, Lee S, Kim DH, Kim S, and Hwang I (2011) Both the hydrophobicity and a positively charged region flanking the C-terminal region of the transmembrane domain of signal anchored proteins play a critical role in determining their targeting specificity to ER and endosymbiotic organelles in plant cells. Plant Cell 23:1588-15607. 100. Zwiewka M, Feraru E, Moller B, Hwang I, Feraru M, Kleine-Vehn J, Weijers D, and Friml J (2011) The AP-3 adaptor complex is required for vacuolar function in Arabidopsis. Cell Research, 21:1711-1722. 101. Kim DH, Xu ZY, Na YJ, Yoo YJ, Lee J, Sohn EJ, and Hwang I (2011) Small heat shock protein Hsp17.8 functions as an AKR2A cofactor in the targeting of chloroplast outer membrane proteins in Arabidopsis. Plant Physiol. 157:132-146. 102. Lee MH, Jung C, Lee J, Kim SY, Lee Y, and Hwang I (2011) An arabidopsis prenylated Rab acceptor 1 isoform, AtPRA1.B6, displays differential inhibitory effects on anterograde trafficking of proteins at the endoplasmic reticulum. Plant Physiol. 157,645-658. 103. Jung C, Lee G-J, Jang M, Lee M, Lee J, Kang H, Sohn EJ, and Hwang I (2011) Identification of sorting motifs of AtFruct4 for trafficking from the ER to the vacuole through the Golgi and PVC. Traffic 12, 1774-1792. 104. Xu ZY, Lee KH, Dong T, Jeong JC, Jin JB, Kanno Y, Kim DH, Kim SY, Seo M, Bressan RA, Yun DJ, and Hwang I (2012) A vacuolar -Glucosidase homolog that possesses glucose-conjugated abscisic acid hydrolyzing activity plays an important role in osmotic stress responses in Arabidopsis. Plant Cell 24:2184-2199. 105. Song K, Jang M, Kim SY, Lee G, Lee GJ, Kim D, Cho W, and Hwang I (2012) An A/ENTH domain-containing protein functions as adaptor for clathrin-coated vesicles on the growing cell plate in Arabidopsis root cells. Plant Physiol. 159:1013-1025. 106. Han YJ, Cho KC, Hwang OJ, Choi YS, Shin AY, Hwang I, and Kim JI (2012) Overexpression of an Arabidopsis -glucosidase gene enhances drought resistance with dwarf phenotype in creeping bentgrass. Plant Cell Rep. 31:1677-1686. 107. Denecke J, Aniento F, Frigerio L, Hawes C, Hwang I, Mathur J, Neuhaus J-M, and Robinson DG (2012) Secretory pathway research: The more experimental systems the better. Plant Cell 24; 1316-1326. 108. Kim H, Lee SB, Kim HJ, Min MK, Hwang I, and Suh MC (2012) Characterization of glycosylphosphatidylinositol-anchored lipid transfer protein 2 (LTPG2) and overlapping function between LTPG/LTPG1 and LTPG2 in cuticular wax export or accumulation in Arabidopsis thaliana. Plant Cell Physiol. 53:1391-1403. 109. Lee S, Lee DW, Yoo Y-J, Duncan O, Oh YJ, Lee YJ, Lee G, Whelan J, and Hwang I (2012) Mitochondrial targeting of the Arabidopsis F1-ATPase -subunit via multiple compensatory and synergistic presequence motifs. Plant Cell 24:5037-5057. 110. Kang H, Kim SY, Song K, Sohn EJ, Lee YJ, Lee DW, Hara-Nishimura I, and Hwang I (2012) Trafficking of Vacuolar proteins: the crucial role of Arabidopsis vacuolar protein sorting 29 in recycling vacuolar sorting receptor. Plant Cell 24:5058-5073. 111. Lee YJ, Jang M, Song K, Kang H, Lee MH, Zouhar J, Rojo E, Sohn EJ, and Hwang I (2013) Functional identification of sorting receptors involved in trafficking of soluble lytic vacuolar proteins in vegetative cells of Arabidopsis. Plant Physiol. 161:121-133. 112. Min MK, Jang M, Lee MH, Lee J, Song K, Lee YJ, Hur M-W, Robinson DG, and Hwang I (2013) Recruitment of Arf1-GDP to Golgi by Glo3p-type ArfGAPs is crucial for Golgi maintenance and plant growth. Plant Physiol. 161:676-691. 113. Lee DW, Jung C, and Hwang I (2013) Cytosolic events involved in chloroplast protein targeting. Biochim Biophys Acta. 1833, 245-252. 114. Xu ZY, Kim SY, Hyeon do Y, Kim DH, Dong T, Park Y, Jin JB, Joo SH, Kim SK, Hong JC, Hwang D, Hwang I. (2013) The Arabidopsis NAC transcription factor ANAC096 cooperates with bZIP-type transcription factors in dehydration and osmotic stress responses. Plant Cell. 25:4708-4724. 115. Xu ZY, Kim DH, and Hwang I (2013) ABA homeostasis and signaling involving multiple subcellular compartments and multiple receptors. Plant Cell Rep. 32:807-813. 116. Park M, Song K, Reichardt I, Kim H, Mayer U, Stierhof YD, Hwang I, and Jurgens G (2013) Arabidopsis -adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth. Proc Natl Acad Sci U S A. 110:10318-10323. 117. Di Rubbo S, Irani NG, Kim SY, Xu ZY, Gadeyne A, Dejonghe W, Vanhoutte I, Persiau G, Eeckhout D, Simon S, Song K, Kleine-Vehn J, Friml J, De Jaeger G, Van Damme D, Hwang I, and Russinova E (2013) The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid insenstive1 in Arabidopsis. Plant Cell. 25:2986-2997. 118. Kim SY, Xu ZY, Song K, Kim DH, Kang H, Reichardt I, Sohn EJ, Friml J, Juergens G, and Hwang I (2013) Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in Arabidopsis. Plant Cell. 25:2970-2985. 119. Kim DH, Xu ZY, and Hwang I (2013) AtHSP17.8 overexpression in transgenic lettuce gives rise to dehydration and salt stress resistance phenotypes through modulation of ABA-mediated signaling. Plant Cell Rep. 32:1953-1963. 120. Kim DH, and Hwang I (2013) Direct targeting of proteins from the cytosol to organelles: the ER versus endosymbiotic organelles. Traffic. 14:613-621. 121. Kim JH, Lee MJ, Hwang I, and Hwang HJ. (2013) In-cell protease assay systems based on trans-localizing molecular beacon proteins using HCV protease as a model system. PLoS One 8:e59710. 122. Lee JY, Sarowar S, Kim HS, Kim H, Hwang I, Kim YJ, and Pai HS (2013) Silencing of Nicotiana benthamiana Neuroblastoma-Amplified Gene causes ER stress and cell death. BMC Plant Biol. 13:69. 123. Lee MH, Lee Y, and Hwang I (2013) In vivo localization in Arabidopsis protoplasts and root tissue. Methods Mol Biol. 1043:113-20. 124. Kim Y, Lee G, Jeon E, Sohn EJ, Lee Y, Kang H, Lee DW, Kim DH, and Hwang I (2014) The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana. Nucleic Acids Res. 42:485-98. 125. Dong T, Xu ZY, Park Y, Kim DH, Lee Y, and Hwang I (2014) Abscisic acid uridine diphosphate glucosyltransferases play a crucial role in abscisic acid homeostasis in Arabidopsis. Plant Physiol. 165:277-289. 126. Dong T, and Hwang I (2014) Contribution of ABA UDP-glucosyltransferases in coordination of ABA biosynthesis and catabolism for ABA homeostasis. Plant Signal Behav. 9. pii: e28888. 127. Lee J, Kim DH, and Hwang I (2014) Specific targeting of proteins to outer envelope membranes of endosymbiotic organelles, chloroplasts, and mitochondria. Front Plant Sci. 5:173. 128. Kim DH, Park M-J, Gwon GH, Silkov A, Xu Z-Y, Yang EC, Song S, Song K, Yoon HS, Honig B, Cho W, Cho Y, and Hwang I (2014) Chloroplast targeting factor AKR2 evolved from an ankyrin repeat domain coincidentally binds two chloroplast lipids. Dev. Cell 30:598-609. 129. Lee M H, and Hwang I (2014) Adaptor proteins in protein trafficking between endomembrane compartments in plants. J. Plant Biol., 57: 265-273. 130. Kang H, and Hwang I (2014) Vacuolar sorting receptor-mediated trafficking of soluble vacuolar proteins in plant cells. Plants 3, 392-408. 131. Lee G, Na YJ, Yang BG, Choi JP, Seo YB, Hong CP, Yun CH, Kim DH, Sohn EJ, Kim JH, Sung YC, Kim YK, Jang MH, and Hwang I (2015) Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection. Plant Biotechnol J. 131:62-72. 132. Kim JH, Oh Y, Yoon H, Hwang I, and Chang YS (2015) Iron nanoparticle-induced activation of plasma membrane H(+)-ATPase promotes stomatal opening in Arabidopsis thaliana. Environ Sci Technol. 49:1113-1119. 133. Kim DH, Lee J-E, Xu Z-Y, Geem KR, Park JW, and Hwang I (2015) Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nature Comm. 6:6843. 134. Oh YJ, and Hwang I (2015) Targeting and biogenesis of transporters and channels in chloroplast envelope membranes: Unsolved questions. Cell Calcium. 58:122-130. 135. Lee DW, Woo S, Geem KR, and Hwang I (2015) Sequence motifs in transit peptides act as independent functional units and can be transferred to new sequence contexts. Plant Physiol. 169:471-484. 136. Dong T, Park Y, and Hwang I (2015). Abscisic acid: biosynthesis, inactivation, homoeostasis and signalling. Essays In Biochemistry 58 29-48. 137. Ahn HK, Kang YW, Lim HM, Hwang I, and Pai HS (2015) Physiological Functions of the COPI Complex in Higher Plants. Mol Cells 38:866-875. 138. Oh YJ, Kim H, Seo SH, Hwang BG, Chang YS, Lee J, Lee DW, Sohn EJ, Lee SJ, Lee Y, and Hwang I (2016) Cytochrome b5 reductase 1 triggers serial reactions that lead to iron uptake in plants. Mol Plant 9:501-513. 139. Yoo YJ, Lee HK, Han W, Kim DH, Lee M, Jeon J, Lee DW, Lee J, Lee Y, Lee J, Kim JS, Cho Y, Han JK, and Hwang I (2016) Interactions between transmembrane helices within monomers of the aquaporin AtPIP2;1 play a crucial role in tetramer formation. Mol Plant 6:1004-1017. 140. Lee DW, Kim SJ, Oh YJ, Choi B, Lee J, and Hwang I (2016) Arabidopsis BAG1 functions as a cofactor in Hsc70-mediated proteasomal degradation of unimported plastid proteins. Mol Plant 9:1428-1431. 141. Park Y, Xu Z-Y, Kim SY, Lee J, Choi B, Lee J, Kim H, Sim H-J, and Hwang I (2016). Spatial regulation of ABCG25, an ABA exporter, is an important component of the mechanism for controlling the cellular ABA levels. Plant Cell 28:2528-2544. 142. Wimmer D, Bohnhorst P, Shekhar V, Hwang I, and Offermann S (2017) Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici. Sci Rep. 7:41187. 143. Ahn G, Kim H, Kim DH, Hanh H, Yoon Y, Singaram I, Wijesinghe KJ, Johnson KA, Zhuang X, Liang Z, Stahelin RV, Jiang L, Cho W, Kang BH, and Hwang I (2017). SH3 domain-containing protein 2 plays a crucial role at the step of membrane tubulation during cell plate formation. Plant Cell. 29:1388-1405. 144. Lee MH, Yoo YJ, Kim DH, Hanh NH, Kwon Y, and Hwang I (2017). The Prenylated Rab GTPase Receptor PRA1.F4 Contributes to Protein Exit from the Golgi Apparatus. Plant Physiol. 174:1576-1594. 145. Jung JY, Lee DW, Ryu SB, Hwang I, and Schachtman DP (2017) SCYL2 genes are involved in clathrin-mediated vesicle trafficking and essential for plant growth. Plant Physiol. 175:194-209. 146. Lee DW, Lee J, and Hwang I (2017) Sorting of nuclear-encoded chloroplast membrane proteins. Curr Opin Plant Biol. 40:1-7. 147. Razzak MA, Lee DW, Yoo YJ, and Hwang I (2017) Evolution of rubisco complex small subunit transit peptides from algae to plants. Sci Rep. 7:9279. 148. Lee DW, Yoo YJ, Razzak MA, and Hwang I (2017) Prolines in transit peptides are crucial for efficient preprotein translocation into chloroplasts. Plant Physiol. 176:663-677 149. Kwon Y, Shen J, Lee MH, Geem KR, Jiang L, and Hwang I (2018) AtCAP2 is crucial for lytic vacuole biogenesis during germination by regulating vacuolar protein trafficking. PNAS 115:E1675-E1683. 150. Nguyen HH, Lee MH, Song K, Ahn G, Lee J, and Hwang I (2018). The A/ENTH domain-containing protein AtECA4 is an adaptor protein involved in cargo recycling from the trans-Golgi network/early endosome to the plasma membrane. Mol Plant. 11:568-583. 151. Kang H, Park Y, Lee Y, Yoo YJ, and Hwang I (2018) Fusion of a highly N-glycosylated polypeptide increases the expression of ER-localized proteins in plants. Sci Rep. 8(1):4612. 152. Lee DW and Hwang I (2018) Evolution and design principles of the diverse chloroplast transit peptides. Mol Cells 41: 161-167. 153. Wang M, Lee J, Choi B, Park Y, Sim HJ, Kim H, and Hwang I (2018) Physiological and Molecular Processes Associated with Long Duration of ABA Treatment. Frontiers Plant Science 9, 176. '9;\]^ O P Q R m J ͩoaQh%hOOJPJQJ^Jo(h%hO5OJQJ^Jh%hO5OJPJQJ^Jh%h%5OJPJQJ^Jh%h]OOJQJ^Jh%h@IOJQJ^Jh%h9OJQJ^Jh~HOJQJ^Jh%hr<OJQJ^Jh9OJQJ^Jh%h(OJQJ^Jh%h~HOJQJ^Jh%h~H5OJQJ^J'O] P Q R m J  . dPG$\$gdO XWD`Xgd9 & Fgd9PWD ^`PgdZgdZ      * , . 0 b d   H L զզզyjX#h%h*i5CJOJQJ^JaJh95CJOJQJ^JaJ#h%h%5CJOJQJ^JaJh8d5CJOJQJ^JaJhOOJPJQJ^Jh%h*iOJPJQJ^Jo(h%h%OJPJQJ^Jh%hOOJPJQJ^Jo(h%hOOJQJ^Jh%hr<OJPJQJ^Jh%hOOJPJQJ^J  >=d.gd9gdZgd]O dPG$\$gdO ct }}}}}}}}}}}hV02jhV02Uh%hiOJQJ^JU!h9h9B*OJQJ^Jphh%h]OOJQJ^J+ !"r#[$,%B&$''(w)Z*P+,,--M/0123679:;=>gd9>?@CA^BCF[GRH4IIJRKKLMrN*O4PPVQR~SYTUUYVVWXgd9XYZ~[\n],^^B__`albbctuvwwwwxxyyzz-|.|gd9154. Sohn EJ, Lee Y, Park N, Park M, Kim NH, Park S, Min K, Gu S, Park Y, Song J, An D-j, and Hwang I (2018) Development of plant-produced E2 protein for use as a green vaccine against classical swine fever virus. J Plant Biol 61, 241-252. 155. Razzak MA, Lee JM, Lee DW, Kim JH, Yoon HS, and Hwang I (2019) Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana. Plant Cell Reports 38, 147-159. 156. Geem KR, Kim DH, Lee DW, Kwon Y, Lee J, Kim JH, Hwang I (2019) Jasmonic acid inducible TSA 1 facilitates ER body formation. Plant J. 97, 267-280. 157. Lee J, Kang H, and Hwang I (2018) Studying Protein Import into Chloroplasts Using Protoplasts. J Vis Exp. (142). doi: 10.3791/58441. 158. Islam MR, Kwak JW, Lee JS, Hong SW, Khan MRI, Lee Y, Lee Y, Lee SW, and Hwang I (2018) Cost-effective production of tag-less recombinant protein in Nicotiana benthamiana. Plant Biotechnol J. [Epub ahead of print] 159. Park et al. (2019) Development of recombinant protein-based vaccine against classical swine fever virus in pigs uisng transgnenic Nicotiana benthamiana. Frontiers in Plant Science (In press) 160. Wimmer D, Bohnhorst P, Impe D, Hwang I, Offermann S. (2019) Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts. Plant Cell Rep. 2019 Jan 19. doi: 10.1007/s00299-019-02375-4. [Epub ahead of print] 161. Lee DW, Lee S, Lee J, Woo S, Razzak MA, Vitale A, Hwang I. (2019) Molecular Mechanism of the Specificity of Protein Import into Chloroplasts and Mitochondria in Plant Cells. Mol Plant. 2019 Mar 16. pii: S1674-2052(19)30094-2. doi: 10.1016/j.molp.2019.03.003. [Epub ahead of print] 162. Muthamilselvan T, Kim JS, Cheong G, Hwang I. (2019) Production of recombinant proteins through sequestration in chloroplasts: a strategy based on nuclear transformation and post-translational protein import. Plant Cell Rep. 2019 May 28. doi: 10.1007/s00299-019-02431-z. [Epub ahead of print] Review. 163. Lee MH, Song K-Y, Hwang HJ, Kim JH, and Hwang I(2019) Development of fast and sensitive protocols for the detection of viral pathogens using a small portable convection PCR platform. Molecular Biology Reports. pp 1-5.     .|/|}}}}}}}}}}}gdDigd9 3........)()()()()() 0P|. A!"#$% 8$Dp s02 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@PJ_HmH nHsH tHH`H \(CJOJQJ^J_HaJmH nHsH tH88 ȩ 1$@&5CJ\aJ(A ( 0 } 4BiB \ \4 l4a $k $] L .U . 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